The Moist Chamber Type 834/8 can be part of a simple constant flow perfusion system for testing potential vasoactive compounds, or used to form the core of a combined constant pressure / constant flow perfusion system for more complex perfusion and dose-response regimens. A flexible silicone platform within the chamber doubles as a surgery table for isolation and cannulation of the organ. The cannulation procedure is simplified through the use of the integrated Mini Ball Joint positioning system and purpose-built arterial and venous cannulae. An extra access port is integrated into the base of each cannula for direct dosing upstream of the organ or sampling of the effluate downstream. In addition, several measurement and sample ports are milled through the chamber wall for easy access to the inner chamber. These innovations permit the researcher to measure a range of physiological, pharmacological and metabolic parameters while maintaining the organ in a closed, controlled environment.