Used Microcentrifuge in Centrifuges

Showing all 9 results

  • Thermo Scientific MySpin 6 Microcentrifuge

    Microcentrifuge

    BSIID: 8004432

    Price: $65.00

    Sale!
    Request a quote Make an Offer
  • VWR Mini Centrifuge

    Microcentrifuge

    BSIID: 8004400

    Price: $50.00

    Sale!
    Request a quote Make an Offer
  • Eppendorf 5415C

    Microcentrifuge

    BSIID: 8004353

    Price: $450.00

    Sale! $450.00 $450.00
    Request a quote.
    Request a quote Make an Offer
  • VWR Galaxy Mini

    Microcentrifuge

    BSIID: 8004342

    Price: $50.00

    Sale! $50.00 $50.00
    Request a quote.
    Request a quote Make an Offer
  • VWR Galaxy 16D

    Microcentrifuge

    BSIID: 8004263

    Price: $500.00

    Sale!
    Request a quote Make an Offer
  • VWR Mini Centrifuge

    Microcentrifuge

    BSIID: 8004237

    Price: $50.00

    Sale!
    Request a quote Make an Offer
  • VWR Galaxy Mini

    Microcentrifuge

    BSIID: 8004032

    Price: $75.00

    Sale! $75.00 $75.00
    Request a quote.
    Request a quote Make an Offer
  • Grant-bio PVC-2400

    Microcentrifuge

    BSIID: 8004018

    Price: $349.00

    Sale!
    Request a quote Make an Offer
  • VWR 1207 Microcentrifuge

    Microcentrifuge

    BSIID: 2036908

    Price: $99.00

    Sale!
    Request a quote Make an Offer

Showing all 9 results

Spinning your way through Centrifugation

Centrifugation is one of the most widely applied research technique in biochemistry, cell biology, molecular biology, and clinical settings. A centrifuge spins and separates liquid samples at high speeds and is considered an essential instrument in almost all laboratories. Centrifugal forces (g-force) pushes heavier particles to the sides of the vessel or bottom (for swinging bucket rotors), separating the particles from the liquid.

A few tips are offered when using some of the various types of centrifuge equipment for sale at BioSurplus, from preparative centrifuges to analytical centrifuges, including ultra, micro, mini, industrial, and benchtop.

There are many types of centrifuges for different applications in a lab. These include:

  1. Mini-centrifuge / Microfuge
  2. Small Benchtop
  3. High-Speed
  4. Ultracentrifuge
  5. Separators (industrial scale centrifuge)

How do I know which type of centrifuge to use?

TypeDescriptionMax RCF(x g)Some Applications
Microfuge / Mini-centrifugeTypically for 1-1.5 mL tubes.32,000Quick separation of small samples, plasmid preps, restriction enzyme reactions, small desalting preps
Small BenchtopLow to medium speed centrifugation for various tube sizes as well as plates.25,000Cell separation for viable cell culture. Larger scale plasmid preps, enzyme assay reactions, precipitation procedures
High-SpeedHigh speed centrifugation100,000Bacterial pelleting, supernatant harvesting for cell culture
Ultracentrifuge1) Preparative2) AnalyticalVery high speed centrifugation.300,000Pelleting particles, membranes, viruses, nucleic acids, lipoproteins. Can also be used to determine molecular shape, mass and sedimentation velocity.
Separators1) Decanter2) Disc stackIndustrial centrifuges used in the biopharmaceutical industry.15,000Used in biopharmaceutical fermentation and bioproduction settings for industrial scale cell harvesting, broth clarification, and separation of cell debris.

 

There are many specifications to think about when choosing a centrifuge. To find the right type of centrifuge for your lab features such as speed, rotor types, and temperature should be considered:

Specifications

Centrifuge Type

Mini-centrifugesMicrofugeSmall BenchtopHigh SpeedUltracentrifuge PreparativeUltracentrifuge AnalyticalSeparators
Max RPMMax RCF (x g)600200017,50030,1317,00025,00026,00082,000150,0001,019,00060,000290,000965012,200
RotorsFixed angle, horizontalFixed angle, horizontalFixed angle, swinging bucket, Continuous flowFixed angle, swinging bucket, Continuous flowFixed angle, swinging bucketFixed AngleNo rotor, sample fed directly to bowl
Rotor Adaptermicrotubes, plates, 10-15mL tubes0.2 mL. 1.5mL/2.0mL microtubes, plates, up to 50 mL tubes, platesplates, up to 50 mL tubes, up to 1L bottlesplates, up to 50 mL tubes, up to 1L bottlesmicrotubes, tubes, bottlestubesNA – Bowl capacity up to 70L
Temp RangeRoom Temp, RefrigeratedRoom Temp, RefrigeratedRoom Temp, RefrigeratedRoom Temp, RefrigeratedRefrigerated 0-40degCRefrigerated 0-40degCRefrigerated
Noise LevelSome models have quiet motorSome models have quiet motorSome models have quiet motor, low noise level <58 dBASome models have quiet motor<50dBALow noise>50dBA
Other FeaturesSmall footprint, quick acceleration/deceleration, on some modelsAutomatic rotor recognition, electronic imbalance detection, one-finger lid closure, soft brake functionQuick Run, Choice of different acceleration/braking curves, RCF selection, rotor recognition, electronic imbalance detection, safety lid lockAutomatic rotor recognition, rotor recognition, electronic imbalance detection, safety lid lockRCF/RPM selection, rotor recognition, safety lid lock, electronic imbalance detection,UV detection for determination of molecular shape, mass and sedimentation velocity, quick acceleration/deceleration and no brake featureBiopharma industrial scale cell harvesting, broth clarification and separation of cell debris, separator can be a closed (sanitized) system, separates particles to 0.1 micron

 

Because centrifuges come in all shapes and sizes, and the rotors vary, the universal unit of centrifugation is in centrifugal force in gravities (x g) or RCF. Be sure to use this value in calculating the rpms required for each rotor.

Here are a few important guidelines for operating a centrifuge

These can help prevent damage to the centrifuge and rotor and more importantly prevent possible serious injury to you and others.

  1. Use the correct rotor and make sure it is properly installed.
  2. Make sure your work surface is level and firm. A centrifuge should never be in operation on an uneven work surface.
  3. Balance the load in a rotor. Use total mass/ weight for each tube as a measurement for balancing rather than volume to take into account different density liquids. If it is an identical liquid that you are balancing against, then volume will work just fine.
  4. Stop or unplug a centrifuge if you see excessive shaking. Check to see if your tubes are balanced and that work surface is level. If that’s not the problem, schedule a service call.
  5. Don’t move or bump the centrifuge while it is spinning. Make sure the centrifuge is in a location where this cannot accidentally happen.
  6. Don’t open the lid to a centrifuge while it is spinning.
  7. Wear safety goggles if you are working near an operational centrifuge.
  8. Use the appropriate sample tubes for your rotor. Make sure your tubes are rated to be able to handle the max rcf of your rotor. Special tubes are needed for high force centrifugation.