The StepOne Real-Time PCR system by Applied Biosystems is a versatile Real-Time PCR platform that is ideal for both new and experienced users. This system offers a 48 well configuration for low throughput environments and is designed to provide the utmost in precision over a range of applications.
The optical system of the StepOne is based around three-color recording and is highly sensitive for optimal performance. This LED fluorescence reading capability allows the instrument to deliver precise results in a cost effective manner without sacrificing durability. The unit is compatible with multiple fluorescent based polymerase chain reaction (PCR) reagents, including FAM/SYBR Green, VIC/JOE, and ROX dyes, and can differentiate between two populations of 5,000 and 10,000 template copies of TaqMan assay with 99.7% certainty.
For convenience, users can additionally save data from all filters in every run without the need for a computer or plate set up, and experimental progress can be monitored at each cycle either remotely or directly on the instrument. The LCD touchscreen interface is intuitive and user-friendly, as are the software and protocol wizard tools. By incorporating these features, user operation is quick and easy, and runs can begin even prior to inputting plate information.
Despite its compact footprint, the StepOne has multiple-configuration capability to meet the needs of virtually any laboratory. Its versatility is further exemplified by the ability of the unit to perform a range of both standard and complex genetic analysis research techniques including, but not limited to: SNP genotyping, gene and mRNA expression, standard curve analysis, melt curve analysis, and relative quantitation. With its precision and flexibility, this RT-PCR instrument is an invaluable tool for any laboratory conducting genetic analysis and research.