The ABI PRISM 7000 Sequence Detection System is a complete, real- time PCR system that detects and quantitates nucleic acid sequences. In real-time PCR, cycle-by-cycle detection of accumulated PCR product is made possible by combining thermal cycling, fluorescence detection, and application- specific software in a single instrument. Quantitative results are available immediately after PCR without additional purification or analysis. Real-time, quantitative PCR applications include gene expression and pathogen detection. Post-PCR detection is also available for non-quantitative assays such as allelic discrimination (SNP detection) and plus/minus assays.