Liquid chromatography is a technique used to separate ions from molecules in a sample. There are two main types of liquid chromatography instruments: the HPLC, or high-performance liquid chromatography (formerly known as high-pressure liquid chromatography), and the FPLC, or fast protein liquid chromatography.
At BioSurplus, we carry a variety of pre-owned HPLC and FPLC models from the top manufacturers. In order to help you decide which make and model is best suited to your research needs, we’ve produced a short video and accompanying in-depth article on liquid chromatography instruments.
In the video you’ll find information on HPLCs and FPLCs and their components, as well as the different makes, models and manufacturers we carry in our showrooms and online store.
In the accompanying article, you can also find a detailed description of HPLC and FPLC components, as well as a chart showing the functions of each type of instrument.
Remember, all equipment we sell is acquired from working labs and comes with a 30-day money-back guarantee. Every instrument is checked by our technicians before it arrives at our warehouse, and then again before we ship it to you.
You can also find out more about one of our most popular HPLC models, the Agilent 1100, on our website.
See below for a transcription of the video:
“The two main types of liquid chromatography instruments are FPLC, or fast protein liquid chromatography and HPLC, or high performance liquid chromatography, also known as high-pressure liquid chromatography.
You can buy either an FPLC or HPLC based on your application. The FPLCs are used for separating out proteins and they preserve the structures of those proteins and operate at lower pump pressure than you would find on an HPLC.
If you’re separating out a mixture of ions you can use an HPLC. HPLCs and FPLCs have the same basic components. A pump, an injector, a column, a detector and the fraction collector.
Samples can also be automated through an auto sampler which allows you to get higher throughput out of your FPLC or HPLC system. For HPLCs we carry Agilent, Shimadzu, Waters and other major manufacturers. For FPLCs we carry GE’s AKTAs Bio-Rad’s FPLCs and many more.”